Journal: Advances in Wound Care

Article Title: Skin Microcolumns as a Source of Paracrine Signaling Factors

doi: 10.1089/wound.2019.1045

Figure Lengend Snippet: MSTC-conditioned media induced endothelial tube formation by HUVECs, but control media did not ( A , each image width corresponds to 1.2 mm). Number of junctions (B) , total tube length (C) , and average tube length (D) were all significantly increased in response to the MSTC-conditioned media. Mean ± 95% CI. * p < 0.05, ** p < 0.005, n = 4. HUVEC, human umbilical vein endothelial cell. Color images are available online.

Article Snippet: Human umbilical vein endothelial cells (HUVECs, purchased from ATCC) were cultured and maintained in Medium 200PRF supplemented with low serum growth supplement (Thermo Fisher Scientific).

Techniques: Control

Journal: Advances in Wound Care

Article Title: Skin Microcolumns as a Source of Paracrine Signaling Factors

doi: 10.1089/wound.2019.1045

Figure Lengend Snippet: Partial list of soluble proteins released by micro skin tissue columns that are known to significantly impact the wound healing process

Article Snippet: Human umbilical vein endothelial cells (HUVECs, purchased from ATCC) were cultured and maintained in Medium 200PRF supplemented with low serum growth supplement (Thermo Fisher Scientific).

Techniques: Migration

Journal: Biofabrication

Article Title: Sheet-based extrusion bioprinting: a new multi-material paradigm providing mid-extrusion micropatterning control for microvascular applications

doi: 10.1088/1758-5090/ad30c8

Figure Lengend Snippet: (A) Fluorescent microscopy on day 1 shows living cells illuminated green around a vacant channel. (B)–(D) Antibody tags label pericytes (hPCs) green and endothelial cells (HUVECs) blue within combined group samples. (B) An entire cell-laden sheet is visible. Cell-laden channels toward the bottom have begun to break off, likely due to 7-day hydrogel degradation accelerated by endpoint immunohistochemical processing reagents. (C) hPCs appear to have seeded with more efficiency than HUVECs, suggesting that interspersing HUVECs within the fugitive ink (‘endothelial seeding method’) before printing was inefficient compared to interspersing cells within the solidifying hydrogel ink. However, both cells are viable while positioned around a vacant channel (D) Side-view of sheet showing visibility of vacant channels that can be hindered from a top-down viewing angle. (E) Cell viability in hydrogel sheets containing both cell types was measured above 90% on both days, although viability in the hPC populations was not measured independently. Viability was lower in the HUVEC-only group, likely attributed to the apparent low-efficiency of the endothelial seeding method. (F) Relative cell numbers were significantly higher in the combined groups, suggesting again that cell numbers were significantly lower in the HUVEC populations due to the inefficient endothelial seeding method, thus contributing to lower HUVEC viability in isolation.

Article Snippet: EndoGRO human umbilical vein endothelial cells (HUVECs) and human brain vascular pericytes (hPCs) were obtained from Millipore Sigma (Burlington, MA, USA) and iXCells Biotechnologies (San Diego, CA, USA) at passage 1 and 2, respectively.

Techniques: Microscopy, Immunohistochemical staining, Isolation